ProtiFi - Precision Medicine Solutions/Service Company
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ProtiFi: Tearing Down Barriers to Make Omics Breakthroughs

Dr. John Wilson, PhD, Founder and CEO, ProtiFiDr. John Wilson, PhD, Founder and CEO, ProtiFi
Going beyond the static biological blueprints probed by genomics, the broader world of omics, which includes transcriptomics, proteomics, metabolomics, lipidomics, and glycomics, opens up new realms of insightful information. It enables detailed, nuanced analysis of the biological conditions that lead to and reveal health and diseases. However, as the adage ‘nothing great ever comes easy’ implies, tapping into the vast pool of knowledge offered by multiomics remains an arduous affair for researchers. Take, for instance, proteomics, which seeks to identify and quantify proteins—the building blocks of life—to derive unprecedented clarity into how biological systems function at their foundational, biochemical levels. A prime hurdle that has hindered proteomics is the fact that proteins, unlike their genomics counterparts DNA and RNA, exhibit diverse solubilities; they are not all water soluble. Additionally, proteins are active micro-machines that can themselves actively change their condition during sample collection, storage and processing. This makes proteomics sample collection and processing incredibly difficult resulting in low reproducibility. Indeed, according to published reports, sample preparation is responsible for a whopping 75 percent of the overall variability in proteomics .

Similar sample conundrums hurdle and derail many omics studies. Furthermore, the lack of standardized and holistic multiomics analyses, combined with limitations in data analysis software to understand the resultant data, continue to hinder omics from truly gaining traction despite their game-changing potential in biology, personalized medicine, and healthcare. The omics domain has longed for standardized, one-size-fits-all techniques and approaches that can remove the current bottlenecks to ultimately accelerate biological advancements.

Delivering just that is ProtiFi. “Our solutions span the whole spectrum in omics research, from sample collection and preparation through to process control and chromatography, and extend all the way to data analysis and the early detection of disease. This level of robustness has never been achieved before and it has allowed the pipelines we developed to be installed in research and clinical laboratories, in hospitals, and medical settings,” says Dr. John Wilson, Founder and CEO of ProtiFi.

For proteomics, the company provides the S-Trap™, which imparts standardization, simplicity, reproducibility, and automation into sample preparation. Using powerful detergents applicable to all samples, detergents powerful enough to liquefy, say, an entire mouse heart, S-Traps™ seamlessly extracts proteins and removes contaminants like salts, detergents, and buffers. “This has been absolutely transformative because now, finally, labs are able to use the same technique for different samples and get the same outcome,” adds Wilson. For multiomics sample preparation, ProtiFi made the Si-Trap™—the world’s first and only multiomics sample preparation system. It produces multiple fractions for different omics analyses from a single sample.

Upping the sample preparation game even higher, ProtiFi has partnered with Tecan to automate omics workflows. The Tecan A200is affordable automation that enables compact benchtop sample preparation to guarantee process reproducibility and uniformity. “After you place sample in and press the go button, the system singlehandedly does protein concentration and capture, and cleaning and digestion, and delivers the samples ready to be put on a mass spectrometer.


Our solutions span the whole spectrum in omics research, from sample collection and preparation through to process control and chromatography, and extend all the way to data analysis and the early detection of disease

With the Si-Trap, you get lipid metabolites, nucleic acids, glycans, and proteins. With the S-Trap, you can do reproducible proteomics on any sample” explains Wilson. ProtiFi also brings to the table, Tryp-N™, an engineered protease that provides simplified spectra and increases sensitivity in MRM assays.

Because lack of standardization and accessibility of data analysis has also prevented omics approaches from making progress, ProtiFi developed data analysis solutions. Their product SimpliFi™, a first-of-its-kind data-to-meaning engine, enables researchers to bridge the gap between omics data and clinical relevance. SimpliFi runs on massive GPU clusters, which brings immense scalability, and allows analysis of humongous datasets even via smartphones. As the name suggests, the SimpliFi interface is specifically designed to allow the broader researcher community, which may not have extensive omics knowledgeable, to glean insights from their data. “By example a bone biologist, who perhaps only heard of proteomics, can use our sample preparation solutions then use SimpliFi to and gain full understanding of that biology,” Wilson explains.

ProtiFi solutions reduce sample preparation data variability to less than ten percent. This, in conjunction with SimpliFi’s prowess, imparts to researchers the ability to full understanding of their studies, complete with confidence intervals, using non-parametric approaches that eliminate false negatives and false positives due to unmet assumptions. “We have made omics analyses simply work, and that allows scientists, researchers and medical professionals to bring precision omics to life. That’s actually our tagline and it means both breathing life into unkept promises and bringing omics techniques to peoples’ lives,” expounds Wilson.

In a nutshell, ProtiFi leaves no stone unturned in its quest to standardize and democratize omics innovations. The company has bought renowned subject matter experts to its business advisory board; among them is Prof. Dr. Nikolai Slavov, Professor at Northeastern University, and Prof. Dr. Rebekah Gundry: “Their knowledge and skills will help omics reach the next level to make real and tangible impacts on people’s health and wellness,” adds Dr. Wilson.

ProtiFi is an integral member of the omics community and enters into strategic partnerships with industry and academia to develop and offer solutions to all omics bottlenecks. By example, they partnered with PHCbi to provide researchers reliable solution for sample storage, Cell Signaling for PTM analysis and for automation Tecan which specializes in developing, producing, and distributing automated workflow solutions for laboratories in the life sciences sector.

Having established itself as a multi-pronged omics first mover, the future is bright for ProtiFi. The company has indeed come a long way from its humble beginnings in Dr. Wilson’s garage to where it is strategically positioned in the market today.

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Top 10 Precision Medicine Solution Companies - 2021
ProtiFi

Company
ProtiFi

Headquarters
Farmingdale, NY

Management
Dr. John Wilson, PhD, Founder and CEO , ProtiFi

Description
ProtiFi develops and provides innovative solutions to study proteins, metabolites, lipids, and glycans. For proteomics, the company provides the S-Trap™, which imparts standardization, simplicity, reproducibility, and automation into sample preparation. Using powerful detergents applicable to all samples, detergents powerful enough to liquefy, say, an entire mouse heart, S-Traps™ seamlessly extracts proteins and removes contaminants like salts, detergents, and buffers